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Título

Influence of chemical and freezing fixation methods in the freeze-fracture of stratum corneum

AutorLópez Serrano, Olga; López-Iglesias, Carmen; Cócera, Mercè; Walther, P.; Parra Juez, José Luis; Maza, Alfons de la
Palabras claveChemical fixation
High-pressure freezing fixation
Stratum corneum
High-resolution low-temperature SEM
Transmission electron microscopy
Freeze-fracture
Fecha de publicación4-feb-2004
EditorElsevier
CitaciónJournal of Structural Biology 146(3): 302-309 (2004)
ResumenA comparison between two fixation techniques for freeze-fracture was established. Stratum corneum (SC) samples from pig epidermis were fixed using high-pressure freezing (HPF) and using plunging in propane freezing; the latter after chemical fixation. Then, frozen samples were freeze-fractured, coated with platinum–carbon, and visualized using a high-resolution low-temperature scanning electron microscope and a transmission electron microscope. Our results indicate that the plane of freeze-fracture was different depending on the fixation and freezing methodology used. In the samples frozen by HPF without chemical fixation, the fracture plane laid mainly between the lipid lamellae. However, when chemical fixation and plunging in propane freezing was used, the fracture plane did not show preference to a specific way. Plunging in propane freezing of chemically fixed samples, on the other hand, provides a more homogeneous fracture behaviour. Thus, depending on the methodology used, we can favour a visualization of either lipid or protein domains of the SC. These results could be very useful in future ultrastructural studies in order to facilitate the microscopic visualization and interpretation of the complex images such as those of SC and even of other samples in which different domains coexist.
Descripción8 pages, 7 figures.-- PMID: 15099572 [PubMed].-- Printed version published Jun, 2004.-- Corrigendum published in: Journal of Structural Biology 148(2): 257 (Nov 2004), http://dx.doi.org/10.1016/j.jsb.2004.06.001
Versión del editorhttp://dx.doi.org/10.1016/j.jsb.2004.01.003
URIhttp://hdl.handle.net/10261/18300
DOI10.1016/j.jsb.2004.01.003
ISSN1047-8477 (Print)
1095-8657 (Online)
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