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dc.contributor.author | Santos-Moriano, Paloma | - |
dc.contributor.author | Kidibule, Peter E | - |
dc.contributor.author | Míguez, Noa | - |
dc.contributor.author | Fernández Arrojo, Lucía | - |
dc.contributor.author | Ballesteros Olmo, Antonio | - |
dc.contributor.author | Fernández Lobato, María | - |
dc.contributor.author | Plou Gasca, Francisco José | - |
dc.date.accessioned | 2019-05-31T06:01:57Z | - |
dc.date.available | 2019-05-31T06:01:57Z | - |
dc.date.issued | 2019-04-30 | - |
dc.identifier.citation | Catalysts 9(5): 405 (2019) | - |
dc.identifier.issn | 2073-4344 | - |
dc.identifier.uri | http://hdl.handle.net/10261/182934 | - |
dc.description | © The Author(s). | - |
dc.description.abstract | By controlled hydrolysis of chitosan or chitin with different enzymes, three types of chitooligosaccharides (COS) with MW between 0.2 and 1.2 kDa were obtained: fully deacetylated (fdCOS), partially acetylated (paCOS), and fully acetylated (faCOS). The chemical composition of the samples was analyzed by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and MALDI-TOF mass spectrometry. The synthesized fdCOS was basically formed by GlcN, (GlcN)2, (GlcN)3, and (GlcN)4. On the contrary, faCOS contained mostly GlcNAc, (GlcNAc)2 and (GlcNAc)3, while paCOS corresponded to a mixture of at least 11 oligosaccharides with different proportions of GlcNAc and GlcN. The anti-inflammatory activity of the three COS mixtures was studied by measuring their ability to reduce the level of TNF-α (tumor necrosis factor) in murine macrophages (RAW 264.7) after stimulation with a mixture of lipopolysaccharides (LPS). Only fdCOS and faCOS were able to significantly reduce the production of tumor necrosis factor (TNF)-α at 6 h after stimulation with lipopolysaccharides. | - |
dc.description.sponsorship | This work was supported by the Fundación Ramón Areces (XIX Call of Research Grants in Life and Materials Sciences) and the Spanish Ministry of Economy and Competitiveness (Grants BIO2016-76601-C3-1-R and BIO2016-76601-C3-3-R). We also acknowledge the institutional grant from Ramon Areces Foundation to the Centre of Molecular Biology “Severo Ochoa”. The European Union’s Horizon 2020 program also financed this work (Blue Growth: Unlocking the potential of Seas and Oceans; grant agreement No. 634486; INMARE). We thank the support of the EU COST-Action CM1303 on Systems Biocatalysis. | - |
dc.publisher | Multidisciplinary Digital Publishing Institute | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-1-R | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-3-R | - |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/634486 | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.subject | Biocatalysis | - |
dc.subject | Glycosidases | - |
dc.subject | Chitinases | - |
dc.subject | Chitosanases | - |
dc.subject | Chitosan oligosaccharides | - |
dc.subject | Deacetylation degree | - |
dc.subject | Anti-inflammatory | - |
dc.title | Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity | - |
dc.type | artículo | - |
dc.identifier.doi | 10.3390/catal9050405 | - |
dc.description.peerreviewed | Peer reviewed | - |
dc.relation.publisherversion | https://doi.org/10.3390/catal9050405 | - |
dc.identifier.e-issn | 2073-4344 | - |
dc.date.updated | 2019-05-31T06:01:57Z | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | European Cooperation in Science and Technology | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000921 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
Aparece en las colecciones: | (ICP) Artículos (CBM) Artículos |
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Tailored_Santos_Art2019.pdf | 1,11 MB | Adobe PDF | Visualizar/Abrir |
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