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Tailored Enzymatic Synthesis of Chitooligosaccharides with Different Deacetylation Degrees and Their Anti-Inflammatory Activity

AuthorsSantos-Moriano, P.; Kidibule, P.; Míguez, N.; Fernández-Arrojo, L.; Ballesteros, A.O.; Fernández-Lobato, M.; Plou, F.J.
Issue Date30-Apr-2019
PublisherMultidisciplinary Digital Publishing Institute
CitationCatalysts 9 (5): 405 (2019)
AbstractBy controlled hydrolysis of chitosan or chitin with different enzymes, three types of chitooligosaccharides (COS) with MW between 0.2 and 1.2 kDa were obtained: fully deacetylated (<i>fd</i>COS), partially acetylated (<i>pa</i>COS), and fully acetylated (<i>fa</i>COS). The chemical composition of the samples was analyzed by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and MALDI-TOF mass spectrometry. The synthesized <i>fd</i>COS was basically formed by GlcN, (GlcN)<sub>2</sub>, (GlcN)<sub>3</sub>, and (GlcN)<sub>4</sub>. On the contrary, <i>fa</i>COS contained mostly GlcNAc, (GlcNAc)<sub>2</sub> and (GlcNAc)<sub>3</sub>, while <i>pa</i>COS corresponded to a mixture of at least 11 oligosaccharides with different proportions of GlcNAc and GlcN. The anti-inflammatory activity of the three COS mixtures was studied by measuring their ability to reduce the level of TNF-&alpha; (tumor necrosis factor) in murine macrophages (RAW 264.7) after stimulation with a mixture of lipopolysaccharides (LPS). Only <i>fd</i>COS and <i>fa</i>COS were able to significantly reduce the production of tumor necrosis factor (TNF)-&alpha; at 6 h after stimulation with lipopolysaccharides.
Identifiersdoi: 10.3390/catal9050405
Appears in Collections:Colección MDPI
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