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Title

Tips for the functionalization of nanoparticles with antibodies

AuthorsPolo, Ester; Puertas, Sara; Moros, María; Batalla, Pilar; Guisán, José Manuel ; Fuente, Jesús M. de la; Grazú, Valeria
KeywordsNanoparticles
Ionic adsorption
Covalent immobilization
Immobilization methods
Oriented antibody
Issue Date2013
PublisherHumana Press
CitationMethods in molecular biology 1051: 149-163 (2013)
AbstractMultiple antibody immobilization methodologies have been developed for several applications including affinity chromatography, immunosensing, and drug delivery. Most of them have been carried out without considering the orientation of the antigen binding site of the antibody, or after the chemical modification of the antibody. An efficient immobilization to improve the biological activity of the antibody is one of the key fundamental issues to pursue. A simple and effective methodology for well-oriented covalently immobilization of antibodies on nanoparticles is reported in this chapter.
Publisher version (URL)https://doi.org/10.1007/978-1-62703-550-7_11
URIhttp://hdl.handle.net/10261/182672
DOI10.1007/978-1-62703-550-7-11
ISSN1064-3745
E-ISSN1940-6029
Appears in Collections:(ICP) Artículos
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