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Título: | Glutaraldehyde-mediated protein immobilization |
Autor: | López-Gallego, Fernando CSIC ORCID; Guisán, José Manuel CSIC ORCID ; Betancor, Lorena | Palabras clave: | Protein stabilization Protein immobilization Glutaraldehyde Cross-linking |
Fecha de publicación: | 2013 | Editor: | Humana Press | Citación: | Methods in molecular biology 1051: 33-41 (2013) | Resumen: | In this chapter, we describe different approaches for the utilization of glutaraldehyde in protein immobilization. First, we focus on the covalent attachment of proteins to glutaraldehyde-activated matrixes. We describe conditions for the synthesis of such supports and provide an example of the immobilization and stabilization of fructosyltransferase. We also describe how glutaraldehyde may be used for the cross-linking of protein–protein aggregates and protein adsorbed onto amino-activated matrixes. In these cases, glutaraldehyde bridges either two lysine groups from different proteic molecules or a lysine from the protein structure and an amine group from the support. Examples of cross-linking are given for the immobilization of DAAO on different amino-activated supports. | Versión del editor: | https://doi.org/10.1007/978-1-62703-550-7_3 | URI: | http://hdl.handle.net/10261/182616 | DOI: | 10.1007/978-1-62703-550-7 | ISSN: | 1064-3745 | E-ISSN: | 1940-6029 |
Aparece en las colecciones: | (ICP) Artículos |
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