English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/182058
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

The RNA Polymerase II factor RPAP1 is critical for Mediator-driven transcription and cell identity.

AuthorsLynch, C.H.; Bernad, Raquel; Calvo, Isabel; Nóbrega-Pereira, Sandrina; Ruiz, Sergio; Ibarz, Nuria; Martínez del Val, Nuria; Graña, Osvaldo; Gómez-López, Gonzalo; Andrés-León, Eduardo; Espinosa Angarica, Vladimir; Sol, Antonio del; Ortega, Sagrario; Fernandez-Capetillo, Oscoar; Rojo, Enrique; Muñoz, Javier ; Serrano, Manuel
KeywordsMediator
Transcription
RNA polymerase II
Cell identity
Differentiation
Enhancer
Interactome
Issue DateJan-2018
PublisherCell Press
CitationCell Reports 22 (2): 396-410 (2018)
AbstractThe RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity.
Publisher version (URL)http://dx.doi.org/10.1016/j.celrep.2017.12.062
URIhttp://hdl.handle.net/10261/182058
DOI10.1016/j.celrep.2017.12.062
E-ISSN2211-1247
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
The RNA Polymerase II factor RPAP1....pdfArtículo principal4,67 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.