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Title

PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway

AuthorsEscudero-Paniagua, B. ; Bartolomé, Rubén Álvaro ; Rodríguez, Sandra; de los Ríos, Vivian; Pintado, Laura; Jaén, Marta; Lafarga, Miguel; Fernandez-Aceñero, M. Jesús; Casal, J. Ignacio
KeywordsPAUF/ZG16B
Mitosis
Vesicle trafficking
Wnt/ β-catenin
Colorectal cancer progression
Issue Date17-May-2019
PublisherOxford University Press
CitationCarcinogenesis bgz093 (2019)
AbstractPancreatic adenocarcinoma–upregulated factor (PAUF), also known as ZG16B, was previously found in the secretome of metastatic colorectal cancer cells. Here, we demonstrated the presence of PAUF at the intracellular level and its multiple effects on cancer progression. An initial decline of PAUF expression was observed at early stages of colorectal cancer followed by an increase at the metastastic site. PAUF was located at different cellular compartments: membrane-associated vesicles, endosomes, microtubule-associated vesicles, cell growth cones and the cell nucleus. PAUF loss in two colorectal cancer cell lines caused severe alterations in the cell phenotype and cell cycle, including tetraploidy, extensive genomic alterations, micronuclei, and increased apoptosis. An exhaustive analysis of the PAUF interactome using different proteomic approaches revealed the presence of multiple components of the cell cycle, mitotic checkpoint, Wnt pathway and intracellular transport. Among the interacting proteins we found ZW10, a moonlighting protein with a dual function in membrane trafficking and mitosis. In addition, PAUF silencing was associated to APC loss and increased β-catenin nuclear expression. Altogether, our results suggest that PAUF depletion increases aneuploidy, promotes apoptosis and activates the Wnt/β-catenin pathway in colorectal cancer cells facilitating cancer progression. In summary, PAUF behaves as a multifunctional protein, with different roles in cancer progression according to the extra- or intracellular expression, suggesting a therapeutic value for colorectal cancer.
Description37 p.-6 fig.
Publisher version (URL)https://doi.org/10.1093/carcin/bgz093
URIhttp://hdl.handle.net/10261/182047
DOI10.1093/carcin/bgz093
ISSN0143-3334
E-ISSN1460-2180
Appears in Collections:(CIB) Artículos
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