English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/18201
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 30 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Study of the oxidation of resveratrol catalyzed by polyphenol oxidase. Effect of polyphenol oxidase, laccase and peroxidase on the antiradical capacity of resveratrol

AutorWichers, Harry J.; Espín de Gea, Juan Carlos
Fecha de publicaciónjul-2000
EditorWiley-Blackwell
CitaciónJournal of food biochemistry 24(3): 225-250 (2000)
ResumenThis paper reports the study of the oxidation of resveratrol catalyzed by polyphenol oxidase (PPO) as -well as the effect of PPO, laccase and peroxidase on the antiradical capacity of resveratrol (measured as capacity to scavenge 2,2'-azino-bis (3-ethylbenzthiazoline - 6 - sulfonic acid) radicals (ARTS')). Monophenolase activity of mushroom PPO in the presence of resveratrol showed the characteristic lag period (τ) prior the attainment of the steady state rate (Vss). The Michaelis constant (Km) for the oxidation of resveratrol catalyzed by PPO was 45 ± 2 μM at pH 6.8 and it decreased with pH. However, the maximum steady state rate (Vmax) remained constant with pH. Both τ and Vss depended on pH in a sigmoid way. Kinetic and NMR assays confirmed that this compound is a substrate which fulfilled both kinetic and structural reaction mechanisms of PPO. Neither laccase, nor PPO modified the antiradical capacity of resveratrol. However, resveratrol, in the presence of peroxidase, lost its antiradical capacity. A possible correlation between antiradical capacity of resveratrol and its oxidation status is proposed. This paper tries to increase the knowledge about this promising health-beneficial molecule and to demonstrate that PPO could be involved in the oxidation pathway of resveratrol.
Descripción26 pages, 8 figures, 2 tables.-- Online version published Feb. 2007.
Versión del editorhttp://dx.doi.org/10.1111/j.1745-4514.2000.tb00698.x
URIhttp://hdl.handle.net/10261/18201
DOI10.1111/j.1745-4514.2000.tb00698.x
ISSN0145-8884
Aparece en las colecciones: (CEBAS) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.