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Flavonoids and phenolic acids of sage: Influence of some agricultural factors

AutorAreias, Filipe M.; Valentão, Patricia; Andrade, Paula B.; Ferreres, Federico ; Seabra, Rosa M.
Palabras claveSage (Salvia officinalis)
Phenolic profile
Quality control
Fecha de publicación15-nov-2000
EditorAmerican Chemical Society
CitaciónJournal of Agricultural and Food Chemistry 48(12): 6081-6084 (2000)
ResumenA reversed-phase high-performance liquid chromatography/diode-array detector procedure is proposed for the determination of six phenolic compounds (caffeic acid, luteolin 7-O-glucoside, rosmarinic acid, apigenin, hispidulin, and cirsimaritin) in sage. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS2 (5-μm particle size, 25.0 × 0.46 cm) column. Of the several extractive solvents assayed only ethyl ether, ethyl acetate, and acetone were able to extract all the compounds mentioned. Best resolution was obtained using a gradient of water/phosphoric acid (999:1) and acetonitrile. Ten samples cultivated in two experimental fields (1997−1999) were analyzed and the individual compounds quantified. Four commercially available samples were also analyzed and the results are discussed.
Descripción4 pages, 5 figures, 1 table.
Versión del editorhttp://dx.doi.org/10.1021/jf000440+
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