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dc.contributor.authorNeptuno-Rodríguez, José-
dc.contributor.authorEspín de Gea, Juan Carlos-
dc.contributor.authorAmor, Francisco del-
dc.contributor.authorTudela, José-
dc.contributor.authorMartínez, Vicente-
dc.contributor.authorCerdá, Antonio-
dc.contributor.authorGarcía-Cánovas, Francisco-
dc.date.accessioned2009-10-30T09:32:18Z-
dc.date.available2009-10-30T09:32:18Z-
dc.date.issued2000-04-18-
dc.identifier.citationJournal of Agricultural and Food Chemistry 48(5): 1537-1541 (2000)en_US
dc.identifier.issn0021-8561-
dc.identifier.urihttp://hdl.handle.net/10261/18197-
dc.description5 pages, 6 figures, 1 table.en_US
dc.description.abstractThe partial characterization of an anionic peroxidase in melon fruit is described. Four melon peroxidase (MPX) isoenzymes were detected in crude extracts after isoelectric focusing. The major MPX isoenzyme (pI = 3.7) was partially purified by including hydrophobic and anion-exchange chromatography in the purification scheme. The sample obtained was used to characterize MPX. This peroxidase did not show activity on ascorbic acid but oxidized guaiacol at a high rate, showing an optimum pH of 5.5 when acting on this last reducing substrate. Melon fruits grown under highly saline conditions showed slightly increased levels of this anionic isoenzyme. Kinetic studies using 2,2‘-azinobis(3-ethylbenzothiazolinesulfonic acid) (ABTS) as reducing substrate showed that increased salinity in the growth medium did not modify the kinetic parameters of melon peroxidase on both hydrogen peroxide and reducing substrate.en_US
dc.description.sponsorshipThis work was supported in part by a grant from the Comisión Interministerial de Ciencia y Tecnologia (CICYT), Spain, projects PB98- 0403-C02-01 and AGF95-0135.en_US
dc.format.extent259768 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsclosedAccessen_US
dc.subjectMelonen_US
dc.subjectPeroxidaseen_US
dc.subjectSalinityen_US
dc.subjectPeroxidase isoenzymeen_US
dc.subjectEnzyme kineticsen_US
dc.titlePurification and kinetic characterization of an anionic peroxidase from melon (Cucumis melo L.) cultivated under different salinity conditionsen_US
dc.typeArtículoen_US
dc.identifier.doi10.1021/jf9905774-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1021/jf9905774en_US
dc.identifier.e-issn1520-5118-
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100007273es_ES
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