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Título

Study of the involvement of H+-ATPase and osmotic adjustment in the resistance of Catharanthus roseus suspension cells to salt stress

AutorElkahoui, Salem; Carvajal, Micaela ; Ghrir, Rachid; Limam, Férid
Palabras claveCatharanthus roseus
H+
ATPase
Osmotic adjustment
Osmotic potential
Plasma membrane
Salt stress
Fecha de publicación2-feb-2005
EditorSpringer
CitaciónPlant Cell Organ and Tissue Culture 80(3): 278-294(2005)
ResumenThe salt-induced H+-ATPase activity and osmotic adjustment responses of Catharanthus roseus (L.) G. Don suspension cultures were studied. Cells were treated with 0, 50 or 100mM NaCl for 7days or were maintained for 8 months with 50 mM NaCl (50T cells). Growth, osmotic potential (), ions content, soluble sugars, proline and total amino acids were determined in the sap of control and salt-treated cells. Salinity reduced cell growth and . The higher decrease in the in salt-treated cells was due to higher accumulation of Na+ and Cl–. The levels of organic solutes, such as soluble sugars, free proline and total amino acids, increased with salt treatment. These results suggest that salt-tolerant cells are able to osmotically adjust. Salinity treatments stimulated H+-ATPase activity. Immunodetection of the enzyme showed that the increased activity was due to an increased amount of protein in the plasmalemma. The induction by NaCl, especially at 100 mM NaCl and for 50T cells, could account for the K+ and Cl– uptake but not for higher or lower tolerance.
Versión del editorhttp://dx.doi.org/10.1007/s11240-004-1085-6
URIhttp://hdl.handle.net/10261/18191
DOI10.1007/s11240-004-1085-6
ISSN0167-6857 (Print)
1573-5044 (Online)
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