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Title

Membrane reconstitution of FtsZ-ZipA complex inside giant spherical vesicles made of E. coli lipids: Large membrane dilation and analysis of membrane plasticity

AuthorsLópez-Montero, Iván; López-Navajas, Pilar; Mingorance, Jesús; Vélez, Marisela ; Vicente, Miguel; Monroy, Francisco
KeywordsBacterial division
E. coli
FtsZ
ZipA
Membrane plasticity
Giant vesicles
Issue DateFeb-2013
PublisherElsevier
CitationBiochimica et Biophysica Acta - Biomembranes 1828(2): 687-698 (2013)
AbstractDuring the division process of Escherichia coli, the globular protein FtsZ is early recruited at the constriction site. The Z-ring, based on FtsZ filaments associated to the inner cell membrane, has been postulated to exert constriction forces. Membrane anchoring is mediated by ZipA, an essential transmembrane protein able to specifically bind FtsZ. In this work, an artificial complex of FtsZ–ZipA has been reconstituted at the inner side of spherical giant unilamellar vesicles made of E. coli lipids. Under these conditions, FtsZ polymerization, triggered when a caged GTP analogue is UV-irradiated, was followed by up to 40% vesicle inflation. The homogeneous membrane dilation was accompanied by the visualization of discrete FtsZ assemblies at the membrane. Complementary rheological data revealed enhanced elasticity under lateral dilation. This explains why vesicles can undergo large dilations in the regime of mechanical stability. A mechanical role for FtsZ polymers as promoters of membrane softening and plasticization is hypothesized.
Publisher version (URL)https://doi.org/10.1016/j.bbamem.2012.11.003
URIhttp://hdl.handle.net/10261/181901
DOI10.1016/j.bbamem.2012.11.003
ISSN0006-3002
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