English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/181901
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Membrane reconstitution of FtsZ-ZipA complex inside giant spherical vesicles made of E. coli lipids: Large membrane dilation and analysis of membrane plasticity

AuthorsLópez-Montero, Iván; López-Navajas, Pilar; Mingorance, Jesús; Vélez, Marisela ; Vicente, Miguel; Monroy, Francisco
KeywordsBacterial division
E. coli
Membrane plasticity
Giant vesicles
Issue DateFeb-2013
CitationBiochimica et Biophysica Acta - Biomembranes 1828(2): 687-698 (2013)
AbstractDuring the division process of Escherichia coli, the globular protein FtsZ is early recruited at the constriction site. The Z-ring, based on FtsZ filaments associated to the inner cell membrane, has been postulated to exert constriction forces. Membrane anchoring is mediated by ZipA, an essential transmembrane protein able to specifically bind FtsZ. In this work, an artificial complex of FtsZ–ZipA has been reconstituted at the inner side of spherical giant unilamellar vesicles made of E. coli lipids. Under these conditions, FtsZ polymerization, triggered when a caged GTP analogue is UV-irradiated, was followed by up to 40% vesicle inflation. The homogeneous membrane dilation was accompanied by the visualization of discrete FtsZ assemblies at the membrane. Complementary rheological data revealed enhanced elasticity under lateral dilation. This explains why vesicles can undergo large dilations in the regime of mechanical stability. A mechanical role for FtsZ polymers as promoters of membrane softening and plasticization is hypothesized.
Publisher version (URL)https://doi.org/10.1016/j.bbamem.2012.11.003
Appears in Collections:(CIB) Artículos
(CNB) Artículos
(ICP) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.