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dc.contributor.authorLancelot, Alexandrees_ES
dc.contributor.authorGonzález-Pastor, Rebecaes_ES
dc.contributor.authorConcellón, Albertoes_ES
dc.contributor.authorSierra, Teresaes_ES
dc.contributor.authorMartín-Duque, Pilares_ES
dc.contributor.authorSerrano, José Luises_ES
dc.date.accessioned2019-05-21T11:02:09Z-
dc.date.available2019-05-21T11:02:09Z-
dc.date.issued2017-
dc.identifier.citationBioconjugate Chemistry 28(4): 1135-1150 (2017)es_ES
dc.identifier.issn1043-1802-
dc.identifier.urihttp://hdl.handle.net/10261/181892-
dc.description.abstractIn the search for effective vehicles to carry genetic material into cells, we present here new pseudodendrimers that consist of a hyperbranched polyester core surrounded by amino-terminated 2,2-bis(hydroxymethyl)propionic acid (bis-MPA) dendrons. The pseudodendrimers are readily synthesized from commercial hyperbranched bis-MPA polyesters of the second, third, and fourth generations and third-generation bis-MPA dendrons, bearing eight peripheral glycine moieties, coupled by the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). This approach provides globular macromolecular structures bearing 128, 256, and 512 terminal amino groups, and these can complex pDNA. The toxicity of the three pseudodendrimers was studied on two cell lines, mesenchymal stem cells, and HeLa, and it was demonstrated that these compounds do not affect negatively cell viability up to 72 h. The complexation with DNA was investigated in terms of N-to-P ratio and dendriplex stability. The three generations were found to promote internalizing of pDNA into mesenchymal stem cells (MSCs), and their transfection capacity was compared with two nonviral commercial transfection agents, Lipofectamine and TransIT-X2. The highest generations were able to transfect these cells at levels comparable to both commercial reagents.es_ES
dc.description.sponsorshipThis work was financially supported by the MINECO-FEDER funds (under the projects CTQ2015-70174-P and MAT2015-66208-C3-1-P) and the Gobierno de Aragón-FSE (E04 research groups). A.L. thanks the MECD for his FPU grants (FPU12/05210) R.G. thanks DGA for his PhD grant.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.relationMINECO/ICTI2013-2016/CTQ2015-70174-Pes_ES
dc.relationMINECO/ICTI2013-2016/MAT2015-66208-C3-1-Pes_ES
dc.rightsclosedAccesses_ES
dc.titleDNA transfection to mesenchymal stem cells using a novel type of pseudodendrimer based on 2,2-bis(hydroxymethyl)propionic acides_ES
dc.typeartículoes_ES
dc.identifier.doi10.1021/acs.bioconjchem.7b00037-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1021/acs.bioconjchem.7b00037es_ES
dc.identifier.e-issn1520-4812-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderGobierno de Aragónes_ES
dc.contributor.funderDiputación General de Aragónes_ES
dc.contributor.funderMinisterio de Educación, Cultura y Deporte (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100010067es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003176es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.pmid28256825-
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