Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/181892
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | DNA transfection to mesenchymal stem cells using a novel type of pseudodendrimer based on 2,2-bis(hydroxymethyl)propionic acid |
Autor: | Lancelot, Alexandre; González-Pastor, Rebeca; Concellón, Alberto CSIC ORCID; Sierra, Teresa CSIC ORCID ; Martín-Duque, Pilar; Serrano, José Luis CSIC ORCID | Fecha de publicación: | 2017 | Editor: | American Chemical Society | Citación: | Bioconjugate Chemistry 28(4): 1135-1150 (2017) | Resumen: | In the search for effective vehicles to carry genetic material into cells, we present here new pseudodendrimers that consist of a hyperbranched polyester core surrounded by amino-terminated 2,2-bis(hydroxymethyl)propionic acid (bis-MPA) dendrons. The pseudodendrimers are readily synthesized from commercial hyperbranched bis-MPA polyesters of the second, third, and fourth generations and third-generation bis-MPA dendrons, bearing eight peripheral glycine moieties, coupled by the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). This approach provides globular macromolecular structures bearing 128, 256, and 512 terminal amino groups, and these can complex pDNA. The toxicity of the three pseudodendrimers was studied on two cell lines, mesenchymal stem cells, and HeLa, and it was demonstrated that these compounds do not affect negatively cell viability up to 72 h. The complexation with DNA was investigated in terms of N-to-P ratio and dendriplex stability. The three generations were found to promote internalizing of pDNA into mesenchymal stem cells (MSCs), and their transfection capacity was compared with two nonviral commercial transfection agents, Lipofectamine and TransIT-X2. The highest generations were able to transfect these cells at levels comparable to both commercial reagents. | Versión del editor: | https://doi.org/10.1021/acs.bioconjchem.7b00037 | URI: | http://hdl.handle.net/10261/181892 | DOI: | 10.1021/acs.bioconjchem.7b00037 | ISSN: | 1043-1802 | E-ISSN: | 1520-4812 |
Aparece en las colecciones: | (ICMA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
4
checked on 17-mar-2024
SCOPUSTM
Citations
15
checked on 15-mar-2024
WEB OF SCIENCETM
Citations
14
checked on 22-feb-2024
Page view(s)
219
checked on 18-mar-2024
Download(s)
41
checked on 18-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.