Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/181730
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Optimized butyl butyrate synthesis catalyzed by thermomyces lanuginosus lipase |
Autor: | Martins, Andrea B.; Friedrich, B.; Rodrigues, Rafael C.; García-Galán, Cristina; Fernández-Lafuente, Roberto CSIC ORCID; Ayub, Marco A. Z. | Palabras clave: | Experimental design Enzyme reuse Esterification Butyl butyrate Lipase |
Fecha de publicación: | dic-2013 | Editor: | Wiley-VCH | Citación: | Biotechnology Progress 29(6): 1416-1421 (2013) | Resumen: | Butyl butyrate is an ester present in pineapple flavor, which is very important for the food and beverages industries. In this work, the optimization of the reaction of butyl butyrate synthesis catalyzed by the immobilized lipase Lipozyme TL-IM was performed. n-Hexane was selected as the most appropriate solvent. Other reaction parameters such as temperature, substrate molar ratio, biocatalyst content and added water, and their responses measured as yield, were evaluated using a fractional factorial design, followed by a central composite design (CCD) and response surface methodology. In the fractional design 24-1, the four variables were tested and temperature and biocatalyst content were statistically significant and then used for optimization on CCD. The optimal conditions for butyl butyrate synthesis were found to be 48°C; substrate molar ratio 3:1 (butanol:butyric acid); biocatalyst content of 40% of acid mass. Under these conditions, over 90% of yield was obtained in 2 h. Enzyme reuse was tested by washing the biocatalyst with n-hexane or by direct reuse. The direct reuse produced a rapid decrease on enzyme activity, while washing with n-hexane allowed reusing the enzyme for five reactions cycles keeping approximately 85% of its activity. © 2013 American Institute of Chemical Engineers. | Versión del editor: | https://doi.org/10.1002/btpr.1793 | URI: | http://hdl.handle.net/10261/181730 | DOI: | 10.1002/btpr.1793 | ISSN: | 8756-7938 | E-ISSN: | 1520-6033 |
Aparece en las colecciones: | (ICP) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
21
checked on 15-mar-2024
WEB OF SCIENCETM
Citations
18
checked on 26-feb-2024
Page view(s)
154
checked on 29-mar-2024
Download(s)
25
checked on 29-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.