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dc.contributor.authorMárquez, Augustoes_ES
dc.contributor.authorAymerich, Joanes_ES
dc.contributor.authorDei, Michelees_ES
dc.contributor.authorRodríguez-Rodríguez, Rosaliaes_ES
dc.contributor.authorVázquez-Carrera, Manueles_ES
dc.contributor.authorPizarro-Delgado, Javieres_ES
dc.contributor.authorGiménez-Gómez, Pabloes_ES
dc.contributor.authorMerlos, Ángeles_ES
dc.contributor.authorSerra-Graells, Francesces_ES
dc.contributor.authorJiménez-Jorquera, Ceciliaes_ES
dc.contributor.authorDomínguez, Carloses_ES
dc.contributor.authorMuñoz Berbel, Xavieres_ES
dc.identifier.citationBiosensors and Bioelectronics, V. 136, 2019, Pages 38-46es_ES
dc.description.abstractAt the point of care (POC), on-side clinical testing allows fast biomarkers determination even in resource-limited environments. Current POC systems rely on tests selective to a single analyte or complex multiplexed systems with important portability and performance limitations. Hence, there is a need for handheld POC devices enabling the detection of multiple analytes with accuracy and simplicity. Here we present a reconfigurable smartphone-interfaced electrochemical Lab-on-a-Chip (LoC)with two working electrodes for dual analyte determination enabling biomarkers' selection in situ and on-demand. Biomarkers selection was achieved by the use of electrodepositable alginate hydrogels. Alginate membranes containing either glucose oxidase (GOx)or lactate oxidase (LOx)were selectively electrodeposited on the surface of each working electrode in around 4 min, completing sample measurement in less than 1 min. Glucose and lactate determination was performed simultaneously and without cross-talk in buffer, fetal bovine serum (FBS)and whole blood samples, the latter being possible by the size-exclusion filtration capacity of the hydrogels. At optimal conditions, glucose and lactate were determined in a wide linear range (0–12 mM and 0–5 mM, respectively)and with high sensitivities (0.24 and 0.54 μA cm −2 mM −1 , respectively), which allowed monitoring of Type-1 diabetic patients with a simple dual analysis system. After the measurement, membranes were removed by disaggregation with the calcium-chelator phosphate buffer. At this point, new membranes could be electrodeposited, this time being selective to the same or another analyte. This conferred the system with on-demand biomarkers’ selection capacity. The versatility and flexibility of the current architecture is expected to impact in POC analysis in applications ranging from homecare to sanitary emergencies.es_ES
dc.relationMEC/ICTI2014-2017/TEC 2014-54449-C3-1-Res_ES
dc.subjectElectrochemical electrodeses_ES
dc.subjectGlucose sensorses_ES
dc.titleReconfigurable multiplexed point of Care System for monitoring type 1 diabetes patientses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
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