English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/181444
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Development of a multiplex assay based on chimeric citrullinated peptides as proof of concept for diagnosis of rheumatoid arthritis

AuthorsGarcía-Moreno, Cristina; Gómara, María José; Bleda, María José; Sanmartí, Raimon; Haro Villar, Isabel
KeywordsPeptides
Antibodies
Biomarkers
Rheumatology
Issue Date2-May-2019
PublisherPublic Library of Science
CitationPLoS ONE 14 (5): e0215927
AbstractAnti-citrullinated peptide/protein antibodies (ACPAs) are the most specific serological biomarkers for rheumatoid arthritis (RA). They have both diagnostic and prognostic value, and are related to more aggressive joint disease in RA. However, a single biomarker cannot differentiate RA subtypes. So, simultaneous analysis of target citrullinated peptides, using a multiplex array based on chimeric peptides composed of several domains of human proteins, could be useful. In this work, eight chimeric peptides and the corresponding native arginine-containing control peptides were obtained by solid-phase peptide synthesis. The study included RA and psoriatic arthritis (PsA) patients attending the Rheumatology Unit of the Hospital Clinic in Barcelona, as well as healthy blood donors (BD) at the same hospital. Our main aim was to explore the diagnostic value of the novel multiplex array compared to a commercial ELISA-based ACPA assay in a serum-saving way. Using the combination of the eight chimeric peptide antigens in the multiplex array, 61.4% of the RA cohort were positive for 3 or more peptides; while, the healthy BD and PsA cohorts did not show any reactivity with the tested peptides. These results indicate that we have developed a highly specific multiplex assay based of chimeric citrullinated peptides derived from filaggrin, fibrin, vimentin and human enolase proteins for the detection of ACPAs in a serum-saving way.
Publisher version (URL)https://doi.org/10.1371/journal.pone.0215927
URIhttp://hdl.handle.net/10261/181444
DOI10.1371/journal.pone.0215927
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
Development of a multiplex assay based on chimeric citrullinated peptides.pdf1,56 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.