English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/181142
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Magnetic separation and high reusability of chloroperoxidase entrapped in multi polysaccharide micro-supports

AuthorsGarcía-Embid, Sonia; Renzo, Francesca di; Matteis, Laura de; Spreti, Nicoletta; Fuente, Jesús M. de la
KeywordsChloroperoxidase
Polysaccharides
Enzyme immobilization
Reusability
Magnetic nanoparticles
Micro-supports
Issue Date2018
PublisherElsevier
CitationApplied Catalysis A: General 560: 94-102 (2018)
AbstractEnzyme immobilization on magnetic supports represents a great advantage for the industrial application of enzymatic catalysis since it allows an easy recovery of the catalyst, avoiding any contamination of the product by residual enzyme. Iron oxide nanoparticles are very useful for this purpose. Using a polymer to diminish the interaction between the magnetic cores themselves, can improve the colloidal stability of the support and prevent any interaction with the environment that would affect both support properties and enzyme stability. For this reason, in this work different magnetic micro-supports, based on polydopamine-coated iron oxide nanoparticles with a multi polysaccharide shell, have been developed. These supports have been used to immobilize chloroperoxidase, a very interesting enzyme, able to catalyze many reactions of large-scale interest, but whose application is limited by its sensitivity to reaction conditions. The multi polysaccharide shells of the supports were obtained through a combination of chitosan and alginate. An in-depth analysis of physicochemical and catalytic properties of all the developed magnetic supports is reported. CPO was successfully immobilized with an efficiency of entrapment between 92% and 100% in the case of supports with chitosan in the interior or outer shell respectively. A very good chemical stability of the support under reaction conditions was observed in the case of an interior shell of alginate and an outer coating of chitosan, together with an excellent reusability of the immobilized enzyme, that was recycled to catalyze up to 25 consecutive reaction cycles.
Publisher version (URL)https://doi.org/10.1016/j.apcata.2018.04.029
URIhttp://hdl.handle.net/10261/181142
DOIhttp://dx.doi.org/10.1016/j.apcata.2018.04.029
ISSN0926-860X
Appears in Collections:(ICMA) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf59,24 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.