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Título: | Direct detection of OXA-48 carbapenemase gene in lysate samples through changes in mechanical properties of DNA monolayers upon hybridization |
Autor: | Domínguez, Carmen M. CSIC; Ramos Vega, Daniel CSIC ORCID; Mingorance, Jesús CSIC ORCID; Fierro, José L.G.; Tamayo de Miguel, Francisco Javier CSIC ORCID; Calleja, Montserrat CSIC ORCID | Fecha de publicación: | 2-ene-2018 | Editor: | American Chemical Society | Citación: | Analytical Chemistry - Columbus 90(1): 968–973 (2018) | Resumen: | Carbapenem-resistant Enterobacteriaceae have recently become an important cause of morbidity and mortality due to healthcare-associated infections. Most commonly used diagnostic methods are incompatible with fast and accurate directed therapy. We report here the direct identification of the blaOXA48 gene, which codes for the carbapenemase OXA-48, in lysate samples from Klebsiella pneumoniae. The method is PCR-free and label-free. It is based on the measurement of changes in the stiffness of DNA self-assembled monolayers anchored to microcantilevers that occur as a consequence of the hybridization. The stiffness of the DNA layer is measured through changes of the sensor resonance frequency upon hybridization and at varying relative humidity. | Versión del editor: | http://dx.doi.org/10.1021/acs.analchem.7b04094 | URI: | http://hdl.handle.net/10261/181112 | DOI: | 10.1021/acs.analchem.7b04094 | ISSN: | 0003-2700 | E-ISSN: | 1520-6882 |
Aparece en las colecciones: | (IMN-CNM) Artículos |
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Direct detection_Dominguez.pdf | 491,69 kB | Adobe PDF | Visualizar/Abrir |
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