English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/181017
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Activity-Based Imaging of Acid Ceramidase in Living Cells

AuthorsOrdóñez, Yadira F.; Abad, José Luis; Aseeri, Mazen; Casas, Josefina; Garcia, Virginie; Casasampere, Mireia; Schuchman, Edward H.; Levade, Thierry; Delgado, Antonio; Triola, Gemma; Fabriàs, Gemma
KeywordsCells
Acid ceramidase
Ceramides
Alzheimer’s disease
Cancer
Issue Date28-Apr-2019
PublisherAmerican Chemical Society
CitationJournal of the American Chemical Society 2019
AbstractAcid ceramidase (AC) hydrolyzes ceramides into sphingoid bases and fatty acids. The enzyme is overexpressed in several types of cancer and Alzheimer’s disease, and its genetic defect causes different incurable disorders. The availability of a method for the specific visualization of catalytically active AC in intracellular compartments is crucial for diagnosis and follow-up of therapeutic strategies in diseases linked to altered AC activity. This work was undertaken to develop activity-based probes for the detection of AC. Several analogues of the AC inhibitor SABRAC were synthesized and found to act as very potent (two-digit nM range) irreversible AC inhibitors by reaction with the active site Cys143. Detection of active AC in cell-free systems was achieved either by using fluorescent SABRAC analogues or by click chemistry with an azide-substituted analogue. The compound affording the best features allowed the unprecedented labeling of active AC in living cells.
Publisher version (URL)https://pubs.acs.org/doi/full/10.1021/jacs.8b11687
URIhttp://hdl.handle.net/10261/181017
DOI10.1021/jacs.8b11687
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
Activity-Based Imaging of Acid Ceramidase in Living Cells.docx Embargoed until April 28, 2020Artículo principal1,93 MBMicrosoft Word XMLView/Open    Request a copy
Activity Based Imaging of Acid Ceramidase in Living Cells. Supporting information.docxMaterial suplementario3,08 MBMicrosoft Word XMLView/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.