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Title

Quantum dots for single-pair fluorescence resonance energy transfer in membraneintegrated EF0F1

AuthorsGálvez Buerba, Eva Mª ; Düser, Monika; Börsch, Michael; Wrachtrup, Jörg; Gräber, P.
KeywordsATP synthesis
Membrane-integrated F0F1
Proton transport
Quantum dot
Rotation
Single-pair fluorescence energy transfer (spFRET)
Issue Date1-Oct-2008
PublisherBiochemical Society
CitationBiochemical Society Transactions 36(5): 1017-1021 (2008)
AbstractspFRET (single-pair fluorescence resonance energy transfer) with organic fluorophores has been used to demonstrate rotation of the subunits γ and ε in membrane-integrated FoF1 during proton transport-coupled ATP synthesis. Owing to the high light intensities used in single-molecule spectroscopy, organic fluorophores show a high probability for photobleaching. Luminescent CdSe/ZnS nanocrystals with a hydrophilic shell have been covalently bound to FoF1 either to the stator subunit b or to the rotor subunit c. TIRFM (total internal reflection microscopy) shows that covalent binding of the QD (quantum dot) via cysteine to FoF1 leads to a significant decrease in the blinking probability in the microsecond-to-second time range. This effect allows the observation of subunit movements in an extended time range. If the QD is bound to the rotor subunit c, the fluorescence anisotropy shows fluctuations in the presence of ATP, in contrast with the constant anisotropy observed in the absence of ATP.
Description2 Figuras.
Publisher version (URL)http://dx.doi.org/10.1042/BST0361017
URIhttp://hdl.handle.net/10261/180855
DOI10.1042/BST0361017
ISSN0300-5127
E-ISSN1470-8752
Appears in Collections:(ICB) Artículos
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