English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/180641
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts

AuthorsRial, Carlos; Varela, Rosa M.; Molinillo, José M. G.; López-Ráez, Juan A. ; Macías, Francisco A.
Keywordsparasitic plants
Solanum lycopersicum
Issue Date2019
PublisherJohn Wiley & Sons
CitationPhytochemical Analysis 30: 110- 116 (2019)
AbstractIntroduction: Strigolactones (SLs) are the most representative germination stimulants for seeds of root parasitic plants, and they show activity even at concentrations below 10 M. The low amounts of stimulants produced by the host and their rapid degradability make it crucial to develop analytical methods with very low limits of quantification. Objective: To develop a sensitive and validated analytical method for the simultaneous quantification of seven SLs [7-oxoorobanchyl acetate (1), solanacol (2), orobanchol (4), strigol (5), fabacyl acetate (6), orobanchyl acetate (7), and 5-deoxystrigol (8)]. Methods: SLs were analysed using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), with (±)-GR24 (3) employed as internal standard (IS). Validation was based on selectivity, linearity, precision of the peak areas (repeatability and intermediate precision), detection and quantification limits, and stability. Results: A simple, rapid and reliable UHPLC-MS/MS method has been validated for the routine analysis of seven SLs and has been successfully applied to quantify them in exudates and extracts from tomato roots (Solanum lycopersicum). The limits of quantifications range from 0.05 μg/L for 5-deoxystrigol to 0.96 μg/L for solanacol. Conclusion: The method provides a useful tool for research in all the fields related to SLs, both for studies related to their function as hormones, and signalling molecules in the rhizosphere, without sample preparation required for extracts and root exudates in less than 11 minutes.
Publisher version (URL)https://onlinelibrary.wiley.com/doi/10.1002/pca.2796
Identifiersdoi: 10.1002/pca.2796
issn: 1099-1565
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
2019_Rial_postprint.pdf1,22 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.