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Fusion proteins comprising an aldolase enzyme joined to a maltose binding protein

AuthorsClapés Saborit, Pere ; Hernández Sánchez, Karel ; Joglar Tamargo, Jesús ; Bujons Vilàs, Jordi ; Fessner, Wolf Dieter; Domínguez de María, Pablo
Issue Date23-Nov-2017
CitationWO2017198717 A1
AbstractThe present invention refers to an enzyme consisting of a fusion protein particularly useful as shown through-out the present invention for carrying out the carbon-carbon bond-forming reaction known as the aldol Reaction, preferably for carrying out an aldol reaction by using aldehydes as substrates and preferably pyruvate or a salt thereof, for producing hydroxyketoacids. Said enzyme is made by binding an aldolase to a maltose binding protein. The enzymes display full activity under "highly denaturing" substrate loadings (aldehydes, > 1 M).
Appears in Collections:(IQAC) Patentes
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