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Title

Reactor and microreactor performance and kinetics of the aldol addition of dihydroxyacetone to benzyloxycarbonyl-N-3-aminopropanal catalyzed by D-fructose-6-phosphate aldolase variant A129G

AuthorsSudar, Martina; Blažević, Zvjezdana Findrik; Szekrényi, Anna; Clapés, Pere; Vasić-Rački, Durda
KeywordsFSA
Batch reactor
Kinetic model
Kinetic parameters
Microreactor
Issue Date3-Jul-2019
PublisherTaylor & Francis
CitationChemical Engineering Communications 206 (7): 927-939 (2019)
AbstractD-Fagomine is an iminosugar found in nature that lowers blood glucose peak after meal and reduces fat-induced weight gain and insulin resistance. The immediate precursor of D-fagomine is accessible straightforward via D-fructose-6-phosphate aldolase catalyzed aldol addition of dihydroxyacetone to Cbz-N-3-aminopropanal (Cbz = benzyloxycarbonyl). In this work, the performance and kinetics of the abovementioned reaction catalyzed by FSA A129G variant was studied. The reaction was investigated in two reactors; batch reactor and microreactor, and kinetic parameters were estimated from the steady state experiments in the batch reactor. This enzyme has improved stability, better K m value for Cbz-N-3-aminopropanal and lower retro-aldol activity in comparison with previously studied aldolase variants FSA A129S and A129S/A165G. Mathematical model for both reactors was developed and validated experimentally. © 2018, © 2018 Taylor & Francis Group, LLC.
Publisher version (URL)https://doi.org/10.1080/00986445.2018.1538975
URIhttp://hdl.handle.net/10261/180393
DOI10.1080/00986445.2018.1538975
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