English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/180382
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Ruminal metagenomic libraries as a source of relevant hemicellulolytic enzymes for biofuel production

AuthorsDuque Martín de la Oliva, Estrella; Daddaoua, Abdelali; Fernández Cordero, B.; Udaondo, Zulema; Molina-Santiago, Carlos; Roca, A.; Solano, J.; Molina Alcaide, E.; Segura, Ana; Ramos Martín, Juan Luis
Issue Date2018
CitationMicrobial Biotechnology 11: 781- 787 (2018)
AbstractThe success of second-generation (2G) ethanol technology relies on the efficient transformation of hemicellulose into monosaccharides and, particularly, on the full conversion of xylans into xylose for over 18% of fermentable sugars. We sought new hemicellulases using ruminal liquid, after enrichment of microbes with industrial lignocellulosic substrates and preparation of metagenomic libraries. Among 150 000 fosmid clones tested, we identified 22 clones with endoxylanase activity and 125 with bxylosidase activity. These positive clones were sequenced en masse, and the analysis revealed open reading frames with a low degree of similarity with known glycosyl hydrolases families. Among them, we searched for enzymes that were thermostable (activity at > 50°C) and that operate at high rate at pH around 5. Upon a wide series of assays, the clones exhibiting the highest endoxylanase andb-xylosidase activities were identified. The fosmids were sequenced, and the corresponding genes cloned, expressed and proteins purified. We found that the activity of the most active b-xylosidase was at least 10-fold higher than that in commercial enzymatic fungal cocktails. Endoxylanase activity was in the range of fungal enzymes. Fungal enzymatic cocktails supplemented with the bacterial hemicellulases exhibited enhanced release of sugars from pretreated sugar cane straw, a relevant agricultural residue.
URIhttp://hdl.handle.net/10261/180382
Identifiersdoi: 10.1111/1751-7915.13269
issn: 1751-7907
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
2018_Duque_MB.pdf114,56 kBUnknownView/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.