English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/179994
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption

AuthorsLama, R.; Pereiro, Patricia ; Costa, M. M. ; Encinar, José Antonio; Medina-Gali, Regla María; Lamas, J.; Leiro, J.; Figueras Huerta, Antonio ; Novoa, Beatriz
KeywordsPhilasterides dicentrarchi
Saposin-like proteins
Antimicrobial peptide
Issue Date2018
CitationFish and Shellfish Immunology 82: 190-199 (2018)
AbstractP. dicentrarchi is one of the most threatening pathogens for turbot aquaculture. This protozoan ciliate is a causative agent of scuticociliatosis, which is a disease with important economic consequences for the sector. Neither vaccines nor therapeutic treatments are commercially available to combat this infection. Numerous antimicrobial peptides (AMPs) have demonstrated broad-spectrum activity against bacteria, viruses, fungi, parasites and even tumor cells; an example is Nk-lysin (Nkl), which is an AMP belonging to the saposin-like protein (SAPLIP) family with an ability to interact with biological membranes. Following the recent characterization of turbot Nkl, an expression plasmid encoding Nkl was constructed and an anti-Nkl polyclonal antibody was successfully tested. Using these tools, we demonstrated that although infection did not clearly affect nkl mRNA expression, it induced changes at the protein level. Turbot Nkl had the ability to inhibit proliferation of the P. dicentrarchi parasite both in vivo and in vitro. Moreover, a shortened peptide containing the active core of turbot Nkl (Nkl71-100) was synthesized and showed high antiparasitic activity with a direct effect on parasite viability that probably occurred via membrane disruption. Therefore, the nkl gene may be a good candidate for genetic breeding selection of fish, and either the encoded peptide or its shortened analog is a promising antiparasitic treatment in aquaculture
Description10 pages, 6 figures
Publisher version (URL)https://doi.org/10.1016/j.fsi.2018.08.004
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Turbot_Scophthalmus_2018.pdf593,12 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.