English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/179985
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples

AuthorsSanchis, Ana ; Salvador, Juan Pablo; Campbell, Katrina; Elliott, Christopher T.; Shelver, Weilin L.; Li, Qingxiao; Marco, M. Pilar
Environmental analysis
Fluorescent microarray chip
Multiplexed analysis
Monoclonal antibodies
Issue Date1-Jul-2018
CitationTalanta - the International Journal of Pure and Applied Analyt Chemistry 184: 499-506 (2018)
AbstractThe development of a fluorescent multiplexed microarray platform able to detect and quantify a wide variety of pollutants in seawater is reported. The microarray platform has been manufactured by spotting 6 different bioconjugate competitors and it uses a cocktail of 6 monoclonal or polyclonal antibodies raised against important families of chemical pollutants such as triazine biocide (i.e. Irgarol 1051®), sulfonamide and chloramphenicol antibiotics, polybrominated diphenyl ether flame-retardant (PBDE, i.e. BDE-47), hormone (17β-estradiol), and algae toxin (domoic acid). These contaminants were selected as model analytes, however, the platform developed has the potential to detect a broader group of compounds based on the cross-reactivity of the immunoreagents used. The microarray chip is able to simultaneously determine these families of contaminants directly in seawater samples reaching limits of detection close to the levels found in contaminated areas (Irgarol 1051® 0.19 ± 0,06 µg L−1; sulfapyridine, 0.17 ± 0.07 µg L−1; chloramphenicol, 0.11 ± 0.03 µg L−1; BDE-47, 2.71 ± 1.13 µg L−1; 17β-estradiol, 0.94 ± 0.30 µg L−1 and domoic acid, 1.71 ± 0.30 µg L−1). Performance of the multiplexed microarray chip was assessed by measuring 38 blind spiked seawater samples containing either one of these contaminants or mixtures of them. The accuracy found was very good and the coefficient of variation was < 20% in all the cases. No sample pre-treatment was necessary, and the results could be obtained in just 1 h 30 min. The microarray shows high sample throughput capabilities, being able to measure simultaneously more than 68 samples and screen them for a significant number of chemical contaminants of interest in environmental screening programs. © 2018 Elsevier B.V.
Publisher version (URL)https://doi.org/10.1016/j.talanta.2018.03.036
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples.docx Embargoed until July 1, 2020636,62 kBMicrosoft Word XMLView/Open    Request a copy
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.