English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/179968
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Deciphering the mode of action of pollutants impairing the fish larvae escape response with the vibrational startle response assay

AuthorsFaria, Melissa ; Bedrossiantz, Juliette ; Prats, Eva ; Rovira García, Xavier; Gómez-Canela, Cristian ; Piña, Benjamín ; Raldúa, Demetrio
Startle and habituation
Cholinergic modulation
Issue Date1-Jul-2019
CitationScience of the Total Environment 672: 121-128 (2019)
AbstractThe escape response evoked by vibrational stimuli and its habituation, essential behaviors for fish larvae survival, can be altered by neurotoxic environmental pollutants commonly found in our aquatic ecosystems. In this study we have analyzed the suitability of the Vibrational Startle Response Assay (VSRA) to obtain mechanistic information about the mode of action (MoA) of the chemicals impairing the escape response and its habituation. As a proof of concept, the pathophysiological mechanisms behind the action of two common neurotoxic pesticides, chlorpyrifos-oxon (CPO) and imidacloprid, over their effects on arousal and habituation of the escape response were studied by using pharmacological antagonists of the nicotinic and muscarinic acetylcholine receptors, mecamylamine (MCA) and scopolamine, respectively. Furthermore, potential changes in the neurotransmitter profile were analyzed. Results revealed that whereas the effect of CPO on arousal was mainly mediated by the activation of nAChRs, its effect on habituation was mainly mediated by mAChRs. On the other hand, imidacloprid only affected larvae arousal which was found to be mediated by a cholinergic independent mechanism. No association between behavioral effects on arousal or habituation in affected larvae was found with their corresponding neurotransmitter profile. These results confirm the suitability of VSRA to provide mechanistic information about the potential MoA of neuroactive compounds.
Publisher version (URL)https://doi.org/10.1016/j.scitotenv.2019.03.469
Appears in Collections:(IDAEA) Artículos
Files in This Item:
File Description SizeFormat 
Deciphering the mode of action of pollutants impairing.pdf Embargoed until July 1, 2021Artículo principal856,48 kBAdobe PDFThumbnail
View/Open    Request a copy
Supplementary Material_R.docx Embargoed until July 1, 2021Material suplementario383,56 kBMicrosoft Word XMLView/Open    Request a copy
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.