English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/179544
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

New fluorogenic probes for neutral and alkaline ceramidases

AuthorsCasasampere, Mireia; Bielsa, Núria; Riba, Daniel; Bassas, Laura; Ruijuan, Xu; Cungui, Mao; Fabriàs, Gemma; Abad, José Luis; Delgado, Antonio; Casas, Josefina
KeywordsCancer
Ceramides
Lipids
Ceramidases
Lipase
Fluorogenic
Substrate
Chemical synthesis
Issue Date29-Mar-2019
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Lipid Research (2019)
AbstractNew fluorogenic ceramidase substrates derived from the N acyl modification of our previously reported probes (RBM14) are reported. While none of the new probes were superior to the known RBM14C12 as acid ceramidase substrates, the corresponding nervonic acid amide (RBM14C24:1) is an efficient and selective substrate for the recombinant human neutral ceramidase, both in cell lysates and in intact cells. A second generation of substrates, incorporating the natural 2 (N acylamino) 1,3 diol 4 ene framework (compounds RBM15) is also reported. Among them, the corresponding fatty acyl amides with an unsaturated N acyl chain can be used as substrates to determine ACER1 and ACER2 activities. In particular, compound RBM15C18:1 has emerged as the best fluorogenic probe reported so far to measure ACER1 and ACER2 activities in a 96 well plate format.
Publisher version (URL)10.1194/jlr.D092759
URIhttp://hdl.handle.net/10261/179544
DOI10.1194/jlr.D092759
Appears in Collections:(IQAC) Artículos
Files in This Item:
File Description SizeFormat 
New fluorogenic probes for neutral and alkaline ceramidases-preprint version.pdf3,25 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.