Analysis and quantification of flavonoidic compounds from Portuguese olive ( Olea Europaea L.) leaf cultivars

Abstract

Twenty three samples of 18 Portuguese olive leaf cultivars were analysed by a reversed-phase HPLC/DAD procedure and eight flavonoidic compounds were identified and quantified (luteolin 7,4- O -diglucoside, luteolin 7- O -glucoside, rutin, apigenin 7- O -rutinoside, luteolin 4- O -glucoside, luteolin, apigenin and diosmetin). Luteolin 7,4- O -diglucoside and luteolin 4- O -glucoside were identified by HPLC/DAD/MS/MS - ESI. The studied olive leaf samples showed a common phenolic pattern, in which luteolin 4- O -glucoside was almost always the major compound.