English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/178783
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Chromatin conformation regulates the coordination between DNA replication and transcription

AuthorsAlmeida Rebelo, José; Fernández-Justel, José Miguel ; Santa-María, Cristina; Cadoret, Jean-Charles; Cano-Aroca, Laura; Lombraña, Rodrigo ; Herranz, Gonzalo; Agresti, Alessandra; Gómez, María
Issue Date2018
PublisherNature Publishing Group
CitationNature Communications 9 (2018)
AbstractChromatin is the template for the basic processes of replication and transcription, making the maintenance of chromosomal integrity critical for cell viability. To elucidate how dividing cells respond to alterations in chromatin structure, here we analyse the replication programme of primary cells with altered chromatin configuration caused by the genetic ablation of the HMGB1 gene, or three histone H1 genes. We find that loss of chromatin compaction in H1-depleted cells triggers the accumulation of stalled forks and DNA damage as a consequence of transcription-replication conflicts. In contrast, reductions in nucleosome occupancy due to the lack of HMGB1 cause faster fork progression without impacting the initiation landscape or fork stability. Thus, perturbations in chromatin integrity elicit a range of responses in the dynamics of DNA replication and transcription, with different consequences on replicative stress. These findings have broad implications for our understanding of how defects in chromatin structure contribute to genomic instability.
URIhttp://hdl.handle.net/10261/178783
Identifiersdoi: 10.1038/s41467-018-03539-8
issn: 2041-1723
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
GómezM_ChromatinConformation.pdf2,57 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.