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Conversation of holm oak (Quercus Ilex L.) by in vitro culture.

AuthorsCernadas, María José; Martínez, María Teresa; Corredoira, Elena ; San José, M. Carmen
KeywordsAxillary shoot proliferation
In vitro conservation
Phytophthora cinnamomi
Issue Date2018
PublisherUniversidad Complutense de Madrid
CitationMediterranean Botany 39(2):97-104 (2018)
AbstractIn vitro culture techniques are used to propagate tree species, as well as to conserve the species in the short and long term. In the present study, in vitro propagation and conservation of holm oak (Quercus ilex L.) were successfully achieved using juvenile material. Mature acorns were germinated under controlled conditions of moisture and temperature, and 3-month-old seedlings were used as sources of explants for culture initiation. Micropropagation via axillary bud proliferation was achieved by culturing shoots in a vertical position on Woody Plant Medium containing different cytokinins and/or concentrations, which were changed every 2 weeks over a 6-week multiplication cycle, as follows: 0.1 mg L-1 benzyladenine (BA) for the first 2 weeks, 0.05 mg L-1 BA for the next 2 weeks, and 0.01 mg L-1 BA plus 0.1 mg L-1 zeatin for the last 2 weeks. Acceptable rooting rates were obtained by culturing microcuttings in Murashige & Skoog medium with half-strength macronutrients supplemented with 3 or 5 mg L-1 indole-3-butyric acid (IBA) in combination with 0.1 mg L-1 naphthalene acetic acid (NAA) for 15 days and subsequent transfer to auxin-free medium for 4 weeks. es Conservación de Quercus ilex L. mediante cultivo in vitro Resumen Las técnicas de cultivo in vitro permiten la propagación de las especies leñosas, así como su conservación a corto y largo plazo. En este trabajo se logró, con éxito, la propagación y la conservación in vitro de encina (Quercus ilex L.) partiendo de material juvenil. Los explantos utilizados para el establecimiento in vitro se obtuvieron a partir de plántulas de 3 meses procedentes de bellotas germinadas bajo condiciones controladas de humedad y temperatura. Los brotes establecidos in vitro se micropropagaron vía proliferación de yemas axilares cultivándolos en posición vertical en medio Woody Plant Medium durante 6 semanas con transferencias a medio fresco cada 2 semanas donde se modifica el tipo de citoquinina y su concentración, como sigue: 0,1 mg L-1 benciladenina (BA) durante las 2 primeras semanas, transferencia a 0,05 mg L-1 BA las 2 semanas siguientes, y por último se transfieren a 0,01 mg L-1 BA más 0,1 mg L-1 zeatina durante 2 semanas más, hasta completar
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