Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/178399
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Overexpression of thioredoxin m in tobacco chloroplasts inhibits the protein kinase STN7 and alters photosynthetic performance |
Autor: | Ancín, María CSIC ORCID ; Fernández San Millán, Alicia CSIC ORCID; Larraya, Luis; Morales Iribas, Fermín CSIC ORCID; Veramendi, Jon CSIC ORCID ; Aranjuelo, Iker CSIC ORCID ; Farrán, Inmaculada CSIC ORCID | Palabras clave: | LHCII phosphorylation Nicotiana tabacum photosynthesis protein complex thioredoxin thylakoid membrane |
Fecha de publicación: | ene-2019 | Editor: | Oxford University Press | Citación: | Journal of Experimental Botany 70 (3): 1005-1016 (2019) | Resumen: | The activity of the protein kinase STN7, involved in phosphorylation of the light-harvesting complex II (LHCII) proteins, has been reported as being co-operatively regulated by the redox state of the plastoquinone pool and the ferredoxin–thioredoxin (Trx) system. The present study aims to investigate the role of plastid Trxs in STN7 regulation and their impact on photosynthesis. For this purpose, tobacco plants overexpressing Trx f or m from the plastid genome were characterized, demonstrating that only Trx m overexpression was associated with a complete loss of LHCII phosphorylation that did not correlate with decreased STN7 levels. The absence of phosphorylation in Trx m-overexpressing plants impeded migration of LHCII from PSII to PSI, with the concomitant loss of PSI–LHCII complex formation. Consequently, the thylakoid ultrastructure was altered, showing reduced grana stacking. Moreover, the electron transport rate was negatively affected, showing an impact on energy-demanding processes such as the Rubisco maximum carboxylation capacity and ribulose 1,5-bisphosphate regeneration rate values, which caused a strong depletion in net photosynthetic rates. Finally, tobacco plants overexpressing a Trx m mutant lacking the reactive redox site showed equivalent physiological performance to the wild type, indicating that the overexpressed Trx m deactivates STN7 in a redox-dependent way. | Descripción: | 12 Pags.- 2 Tabls.- 6 Figs. © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | Versión del editor: | https://doi.org/10.1093/jxb/ery415 | URI: | http://hdl.handle.net/10261/178399 | DOI: | 10.1093/jxb/ery415 | ISSN: | 0022-0957 | E-ISSN: | 1460-2431 |
Aparece en las colecciones: | (EEAD) Artículos (IDAB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
MoralesF_JExpBot_2018.pdf | 1,42 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
11
checked on 07-mar-2024
SCOPUSTM
Citations
22
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
20
checked on 29-feb-2024
Page view(s)
313
checked on 18-abr-2024
Download(s)
211
checked on 18-abr-2024