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Lymphoblasts as potential peripheral biomarkers of Alzheimer's disease and the protective role of resveratrol

AuthorsSenserrich, Júlia
AdvisorSanfeliu, Coral CSIC ORCID
KeywordsAlzheimer disease
Oxidative stress
Issue Date10-Sep-2018
PublisherUniversidad Rovira i Virgili
CSIC - Instituto de Investigaciones Biomédicas de Barcelona (IIBB)
AbstractAlzheimer’s disease (AD) is the most common neurodegenerative disorder and first cause of dementia all over the world. It is characterized by a progressive neuronal loss. The main risk factor for developing AD is the age and it is known that there are shared pathological changes between them. Major pathological hallmarks include extracellular deposits of amyloid protein and intracellular deposits of neurofibrillary tau protein. Moreover, it is though that β-amyloid peptide induce cytotoxicity by the generation of reactive oxygen intermediates, contributing to protein tau’s hyperphosphorylation.
The study used immortalised lymphocytes from patients of sporadic AD and age-matched healthy controls with following aims: First, use real-time PCR to verify the results of several genes expression analysed by array, in a previous study. Second, investigate the potential role of resveratrol, inducing an upregulation of genes involved in the antioxidant cell response and regulating the aging pathway. Finally, establish the different sensitivity between the human immortalized lymphocyte cell lines and human neuroblastoma cell lines, both treated with resveratrol and hydrogen peroxide (H2O2), an oxidative stress inductor.
The current study was observed that gene expression results by PCR did not correlated with those obtained by array, suggesting a variability through individuals. Moreover, aging genes seemed to be more expressed by AD patients, but resveratrol did not induce any effect. Contrary to genes involved in an antioxidant response, which an upregulation induced by resveratrol was clearly demonstrated in most antioxidant genes tested. In addition, it was seen the high toxicity produced by an oxidative stress inductor and the trace that resveratrol 50µM could be the threshold of toxicity for neuronal cells, while lymphoblastomas showed to be more resistant to those compounds.
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