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NMR characterization of differential binding of sugar anomers by lectins

AuthorsEl Biari, Khouzaima; Calle Jiménez, Luis Pablo CSIC; Ribeiro, João P.; Jiménez-Barbero, Jesús CSIC ORCID; Cañada, F. Javier CSIC ORCID ; Dierks, T.; André, Sabine; Gabius, Hans-Joachim
Issue DateSep-2011
PublisherSociedad Española de Bioquímica y Biología Molecular
CitationXXXIV Congreso de la Sociedad Española de Bioquímica y Biología Molecular. Barcelona, 5 al 8 de septiembre de 2011
AbstractReducing carbohydrates can be characterized in two different structures depending of the configuration at the anomeric position of the reducing end. Anomeric selectivity has been observed and studied in enzymes involved in carbohydrate transformations by diverse kinetic approaches since long time ago1, however those approaches are not possible in the case of just binding studies of carbohydrate in solution. Lectins and other carbohydrates receptors do not modified their ligands, thus do not generate time dependent responses feasible of monitoring. Taking into account that free anomers equilibrate in no more than a few hours, many of the available biophysical techniques in solution only provide a mean macroscopic view of the recognition event involving the mixture of anomers in equilibrium. The direct discrimination of the recognition of each anomer is not an easy task, and NMR can be technique of choice because it allows observing independent signals for each anomer in spite of they are in chemical equilibrium exchange. In fact, 13C NMR has been used to show the - anomer preference of bacterial chemotaxis sugar binding proteins 2 and more exotic tritium 3H -NMR has been used to study anomeric preference in maltose binding protein 3. In the present work we have applied STD–NMR strategies, to obtain quantitative information on the individual interaction of each anomer, combined with molecular modeling in order to characterize the differential monosaccharide anomer affinity by some selected lectins.
Description1 p.
Publisher version (URL)https://www.sebbm.es/xxxivcongreso/indexdca8.html?item=2062
Appears in Collections:(CIB) Comunicaciones congresos
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