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Age-related DNA methylation changes in somatic and gonadal tissues of teleost fish (European sea bass)

AuthorsAnastasiadi, Dafni ; Piferrer, Francesc
Issue Date24-Mar-2017
PublisherSocietat Catalana de Biologia
CitationVII Jornada de Cromatina i Epigenètica (2017)
AbstractIn animals, age-related changes in DNA methylation occur. In general, there is global genomic hypomethylation accompanied by CpG-specific hypermethylation as a result of intrinsic and extrinsic influences. Mammalian epigenetic clocks have been suggested and used that estimate the biological age independently of the tissue tested. In fish, ageing studies based on epigenetic changes are scarce, while in fisheries and fish population studies there is a need for accurate estimation of age for which epigenetic biomarkers can be candidates. Here, we suggest CpG loci exhibiting decreasing or increasing methylation with age as potential piscine biomarkers of age. We used individuals from three different age classes comprising the reproductively immature and the senescent phase of a marine teleost fish, the European sea bass, to inquire the methylation status of the regulatory regions of important genes. For this inquiry, we successfully developed a low-cost high-resolution PCR-based NGS protocol applied in 22 genes and 36 samples. We found gene-specific ranges of DNA methylation that were affected by tissue identity and age. Furthermore, we fitted the elastic net regularization paths for generalized linear model in order to identify the minimum CpG number sufficient to predict the fish age. A minimum of 10 CpGs, out of 301 tested in total (3.3%), distributed in 6 genes, were enough to explain the age of the fish. Importantly, CpGs with clear increasing or decreasing tendencies in DNA methylation with age were detected and the youngest fish mean methylation matched with ~1 year old fish from an independent experiment. The sum of the suggested CpGs can serve as a guide to focus the efforts for the development of an accurate intertissue piscine epigenetic clock
DescriptionVII Jornada de Cromatina i Epigenètica organizada por la Secció de Cromatina i Epigenètica de la Societat Catalana de Biologia (SCB) con el Barcelona Chromatin Club (BCC).-- 1 page
Appears in Collections:(ICM) Comunicaciones congresos
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