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Potential role of acetylation on bacterial metabolic pathways

AuthorsCasas, Vanessa ; Abián, Joaquín ; Carrascal, Montserrat
Issue Date16-Jun-2018
CitationXII EuPA Congress (2018)
AbstractBackground: Post-translational modifications (PTMs) of bacterial proteins were almost unknown until the advent of mass spectrometry. Mass spectrometry-based proteomics has revealed that prokaryotes are able to modify their proteins with a high number of PTMs which have influence on bacterial physiology and virulence. Acetylation is the most studied PTM in prokaryotic organisms. Proteins involved in metabolism being the main group of acetylation targets. This modification has been related to the degree of virulence, adaptation to environmental conditions, and persistence in several bacterial species. In this study, acetylomes of Brachyspira hyodysenteriae and B. pilosicoli have been characterized. These species are known gut pathogens; B. hyodysenteriae is the causative agent of swine dysentery and B.pilosicoli causes colonic spirochaetosis in pigs and is responsible for a human form of the disease. Methods: Bacterial acetylomes were prepared by immunoprecipitation with specific anti-acetyl Lys antibody and analysed in an Orbitrap Fusion Lumos¿ Tribrid coupled to a nanoLC system. Raw data were processed with Proteome Discoverer and filtered at 0.1% FDR using Percolator. Localization probability for each site was calculated using ptmRS.. Results: 3142 and 5496 acetylated peptides (3221 and 5579 sites) were identified in B. hyodysenteriae and B. pilosicoli, respectively. The two sets of acetylated proteins were found to be enriched in proteins involved in metabolic pathways and the biosynthesis of secondary metabolites. Conclusions: We have reported a different degree of proteome acetylation in these species, being B. pilosicoli proteome the most acetylated one. We have shown that the main targets of acetylation were proteins involved in metabolism and that the differences observed are reflected in the different composition of the components involved in the Acetyl-CoA/Acetate metabolic pathway.
DescriptionTrabajo presentado en el XII Congreso EuPA: Traslating genomes into biological functions, celebrado en Santiago de Compostela del 16 al 20 de junio de 2018
Appears in Collections:(IIBB) Comunicaciones congresos
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