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Resveratrol upregulates the expression of antioxidant genes inimmortalized AD lymphocytes

AuthorsSenserrich, Júlia; Cosín-Tomás, Marta; Martín Requero, A.; Pallàs, Mercè; Kaliman, Perla ; Sanfeliu, Coral
Issue Date12-Nov-2018
CitationXI Simposi de Neurobiologia (2018)
AbstractAlzheimer’s disease (AD) is the most common neurodegenerative disorder and first cause of dementia all over the world. It is characterized by a progressive neuronal loss. The main risk factor for developing AD is the age. Major pathological hallmarks include extracellular deposits of amyloid protein and intracellular deposits of neurofibrillary tau protein. AD brain also shows alterations developed in aged brain such as oxidative stress and inflammation. The study used immortalized lymphocytes from patients of sporadic AD and age-matched healthy controls (HC) with the following aims: First, analyze gene expression of oxidative stress and aging-related pathways in AD and HC lymphoblast cell lines. Next, investigate the potential role of resveratrol as antiaging and protective agent through the induction of further gene expression changes. Finally, establish the different sensitivity between the human immortalized lymphocyte cell lines and the human neuroblastoma cell line SH-SY5Y to the hormetic agent resveratrol and to oxidative stress inductors. Real-time PCR showed that oxidative stress and aging-related genes were differentially expressed in AD lymphoblasts compared to HC lymphoblasts with increases in CASP1, TXNIP, VPS13C, GPX1, PRDX5, SOD2, and decrease in CCS expression. Resveratrol incubated for 18h at concentration of 10, 20 or 50µM induced a general upregulation of antioxidant and detoxifying genes such as: CAT, NFE2L2, GSTZ1, and CCS, in both HC and AD lymphoblasts. Resveratrol also induced the expression of the anti-aging mitochondrial sirtuin SIRT3. At the concentrations tested, resveratrol was not cytotoxic to lymphoblast or SH-SY5Y neuroblastoma cultures, although the later were more sensitive to oxidative injuries. This study supports that immortalized lymphocytes is a suitable cell system to analyze molecular alterations of pathways common to non-neural and neural cells. Furthermore, resveratrol upregulation of antioxidant genes may contribute to improve physiological processes in aging and AD.
DescriptionTrabajo presentado en el XI Simposi de Neurobiologia: Future technical advances, organizado por la Socitat Catalana de Biologia, en Barcelona, los días 12 y 13 de noviembre de 2018
Appears in Collections:(CIB) Comunicaciones congresos
(IIBB) Comunicaciones congresos
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