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A proteomic approach to identify immunogenic proteins from Brachyspira pilosicoli and B. hyodysenteriae.

AuthorsCasas, Vanessa ; Rodríguez-Asiain, Arantza; Pinto Llorente, Roberto; Abián, Joaquín ; Carrascal, Montserrat
Issue Date2-Apr-2017
CitationProteomic Forum (2017)
Abstract1. Introduction Spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatment against these species, no effective vaccination is available yet.
2. Objectives Identification of suitable candidates for subunit vaccines against SD and PIS diseases through the study of the immunogenic pattern of two B. pilosicoli porcine isolates (Spanish farm isolate OLA-9 and commercial P/43/6/78 strain) and one B. hyodysenteriae isolate (Spanish farm V1).
3. Materials & methods Two B. pilosicoli samples (isolate OLA-9 and ATCC strain P/43/6/78) and one B. hyodysenteriae isolate (isolate V1) were studied. Cell lysates were fractionated using preparative off-gel isoelectrofocusing and the fractions separated by SDS-PAGE. Gels were immunoblotted using pig immune-sera, and the reactive bands were identified by mass spectrometry.
4. Results From the 28 challenge-specific immunoreactive bands detected, 21 produced single protein identifications by MS, while the other 7 shown to contain several major proteins. None of these proteins were detected in control immunoreactive bands. Identifications included 11 proteins from B. hyodysenteriae and 27 from the two B. pilosicoli strains.
5. Conclusion Our immunoproteomics approach demonstrated to be very effective for the characterization of new Brachyspira antigens as it intrinsically takes into account the actual expression levels, molecular characteristics and exposure to the host of the specific antigens that elicit an immune response. This makes the immunoreactive proteins identified unrivalled candidates for vaccines against SD and porcine IS. Some of these proteins had been already tested or suggested as potential vaccine components by other authors, while other are first described. Thus, despite in several cases where immunoreactive bands showed to contain more than one component the actual antigen should be confirmed, the collection of proteins described constitute a unique antigen collection from these bacteria.
DescriptionTrabajo presentado en el Proteomic Forum 2017, celebrado en la University of Potsdam (Germany) del 2 al 6 de abril de 2017
Appears in Collections:(IIBB) Comunicaciones congresos
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