English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/17735
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 21 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly

AutorRódenas, Eduardo; Klerkx, Elke P. F. ; Ayuso, Cristina ; Audhya, Anjon; Askjaer, Peter
Palabras claveCaenorhabditis elegans
Chromatin segregation
Embryogenesis
Mitosis
Nuclear envelope assembly
Nuclear lamina
Nuclear pore complex
Nup35
Nup53
npp-19
Fecha de publicación15-mar-2009
EditorElsevier
CitaciónDevelopmental Biology 327(2): 399-409 (2009)
ResumenNuclear pore complexes (NPCs) are gateways for transport between the nucleus and cytoplasm of eukaryotic cells and play crucial roles in regulation of gene expression. NPCs are composed of multiple copies of not, vert, similar 30 different nucleoporins (nups) that display both ubiquitous and cell type specific functions during development. Vertebrate Nup35 (also known as Nup53) was previously described to interact with Nup93, Nup155 and Nup205 and to be required for nuclear envelope (NE) assembly in vitro. Here, we report the first in vivo characterization of a Nup35 mutation, npp-19(tm2886), and its temperature-dependent effects on Caenorhabditis elegans embryogenesis. At restrictive temperature, npp-19(tm2886) embryos exhibit chromosome missegregation, nuclear morphology defects and die around mid-gastrulation. Depletion of Nup35/NPP-19 inhibits NE localization of Nup155/NPP-8, NPC assembly and nuclear lamina formation. Consequently, nuclear envelope function, including nucleo-cytoplasmic transport, is impaired. In contrast, recruitment of Nup107/NPP-5, LEM-2 and nuclear membranes to the chromatin surface is Nup35/NPP-19-independent, suggesting an uncoupling of nuclear membrane targeting and NPC assembly in the absence of Nup35/NPP-19. We propose that Nup35/NPP-19 has an evolutionary conserved role in NE formation and function, and that this role is particularly critical during the rapid cell divisions of early embryogenesis.
Descripción11 pages, 8 figures, 3 tables.-- PMID: 19146848 [PubMed].-- Available online Dec 30, 2008.
Supporting information (Suppl. figs S1-S5, movies S1-S6) available at: http://dx.doi.org/10.1016/j.ydbio.2008.12.024
Versión del editorhttp://dx.doi.org/10.1016/j.ydbio.2008.12.024
URIhttp://hdl.handle.net/10261/17735
DOI10.1016/j.ydbio.2008.12.024
ISSN0012-1606
Aparece en las colecciones: (CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Rodenas_et_al_081208.pdfAuthors' final manuscript2,4 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.