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Title

AMPK Protein Interaction Analyses by Yeast Two-Hybrid

AuthorsSanz, Pascual ; Viana, Rosa ; Garcia-Gimeno, Maria Adelaida
KeywordsAMPK
Bait plasmid
Prey plasmid
β-Galactosidase
Yeast two-hybrid
Issue Date12-Apr-2018
PublisherHumana Press
CitationMethods in Molecular Biology 1732:143-157 (2018)
AbstractMammalian AMP-activated protein kinase (AMPK) is a Ser/Thr protein kinase that acts as a crucial energy sensor in the cell. Since AMPK plays a key role in a multitude of different pathways in the cell, major efforts have been concentrated to elucidate its signaling network, mainly by the identification of AMPK downstream targets. In this chapter we describe a yeast two-hybrid method for the direct evaluation of the interaction between an AMPK subunit and putative substrates.
DescriptionThis is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-7598-3_9 (15 páginas, 5 figuras)
Publisher version (URL)http://dx.doi.org/10.1007/978-1-4939-7598-3_9
URIhttp://hdl.handle.net/10261/177178
DOI10.1007/978-1-4939-7598-3_9
ISSN1064-3745
E-ISSN1940-6029
Appears in Collections:(IBV) Artículos
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