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http://hdl.handle.net/10261/177127
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Lichtenstein, Mathieu | es_ES |
dc.contributor.author | Carretero, Nina M. | es_ES |
dc.contributor.author | Pérez Soler, Estela | es_ES |
dc.contributor.author | Pulido-Salgado, Marta | es_ES |
dc.contributor.author | Moral-Vico, Javier | es_ES |
dc.contributor.author | Solà, Carme | es_ES |
dc.contributor.author | Casañ Pastor, Nieves | es_ES |
dc.contributor.author | Suñol, Cristina | es_ES |
dc.date.accessioned | 2019-03-04T08:00:21Z | - |
dc.date.available | 2019-03-04T08:00:21Z | - |
dc.date.issued | 2018-09 | - |
dc.identifier.citation | NeuroToxicology 68: 115-125 (2018) | es_ES |
dc.identifier.issn | 0161-813X | - |
dc.identifier.uri | http://hdl.handle.net/10261/177127 | - |
dc.description.abstract | Neural electrode implants are made mostly of noble materials. We have synthesized a nanostructured material combining the good electrochemical properties of iridium oxide (IrOx) and carbon-nanotubes (CNT) and the properties of poly(3,4-ethylenedioxythiophene) (PEDOT). IrOx-CNT-PEDOT charge storage capacity was lower than that of IrOx and IrOx-CNT, but higher than that of other PEDOT-containing hybrids and Pt. Cyclic voltammetry, SEM, XPS and micro-Raman spectroscopy suggest that PEDOT encapsulates IrOx and CNT. In our search for a cell culture platform that could optimize modelling the in vivo environment, we determined cell viability, neuron and astrocyte functionality and the response of astrocytes to an inflammatory insult by using primary cultures of neurons, of astrocytes and co-cultures of both. The materials tested (based on IrOx, CNT and PEDOT, as well as Pt as a reference) allowed adhesion and proliferation of astrocytes and full compatibility for neurons grown in co-cultures. Functionality assays show that uptake of glutamate in neuron-astrocyte co-culture was significantly higher than the sum of the uptake in astrocytes and neurons. In co-cultures on IrOx, IrOx-CNT and IrOx-CNT-PEDOT, glutamate was released by a depolarizing stimulus and induced a significant increase in intracellular calcium, supporting the expression of functional NMDA/glutamate receptors. LPS-induced inflammatory response in astrocytes showed a decreased response in NOS2 and COX2 mRNA expression for IrOx-CNT-PEDOT. Results indicate that neuron-astrocyte co-cultures are a reliable model for assessing the biocompatibility and safety of nanostructured materials, evidencing also that hybrid IrOx-CNT-PEDOT nanocomposite materials may offer larger resistance to inflammatory insults. | es_ES |
dc.description.sponsorship | This work was supported by grants from the Spanish MEC(MAT2011-24363 and MAT2015-65192-R), Instituto de Salud Carlos III(PI 13/1252 and PI15/00033) cofinanced with European Social Funds“A way to build Europe”, Marató TV3 Grant (110130/31), CSIC(201560E053), Generalitat de Catalunya (2017 SGR 106, 2014/1643and Cerca programme) and Severo Ochoa Programme for Centres ofExcellence in R&D (SEV-2015-0496). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2015-65192-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0496 | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Electroactive materials | es_ES |
dc.subject | Iridium oxide-carbon nanotube-conducting polymers | es_ES |
dc.subject | Neural systems | es_ES |
dc.subject | Neural biocompatibility | es_ES |
dc.subject | Neuron-astrocyte co-cultures | es_ES |
dc.subject | Inflammation | es_ES |
dc.title | Biosafety assessment of conducting nanostructured materials by using co-cultures of neurons and astrocytes | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.neuro.2018.07.010 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.neuro.2018.07.010 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Fundació La Marató de TV3 | es_ES |
dc.contributor.funder | Consejo Superior de Deportes (España) | es_ES |
dc.contributor.funder | Generalitat de Catalunya | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100002809 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100008666 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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