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Título: | Quantification of interacting cognate odorants with olfactory receptors in nanovesicles |
Autor: | Sanmartí-Espinal, Marta; Iavicoli, Patrizia CSIC; Calò, Annalisa CSIC; Taulés, Marta; Galvé, Roger CSIC ORCID ; Marco, María Pilar CSIC ORCID CVN ; Samitier, Josep | Palabras clave: | Odors Biosensors Olfactory receptors |
Fecha de publicación: | 1-dic-2017 | Editor: | Springer Nature | Citación: | Scientific Reports 7(1): 17483 (2017) | Resumen: | This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ∼100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors. © 2017 The Author(s). | Versión del editor: | https://doi.org/10.1038/s41598-017-16997-9 | URI: | http://hdl.handle.net/10261/177020 | DOI: | 10.1038/s41598-017-16997-9 |
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Quantification of interacting cognate odorants with olfactory receptors in nanovesicles.pdf | 1,58 MB | Adobe PDF | Visualizar/Abrir |
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