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Título

Esterification of vertebrate-like steroids in the eastern oyster (Crassostrea virginica)

AutorJaner, Gemma; Mesia-Vela, Sonia; Wintermyer, Margy L.; Cooper, Keith R.; Kauffman, Frederick C.; Porte Visa, Cinta
Palabras claveEsterification
DHEA
Estradiol
Crassostrea virginica
Digestive gland
Gonad
Fecha de publicación27-abr-2004
EditorElsevier
CitaciónMarine Environmental Research 58(2-5): 481-484 (2004)
ResumenThe esterification of two model vertebrate steroid hormones –estradiol (E2) and dehidroepiandrosterone (DHEA)– was studied in the oyster Crassostrea virginica. The activity of acyl-CoA:steroid acyltransferase was characterized in microsomal fractions isolated from oyster digestive glands. The apparent Km and Vmax values changed with the fatty acid acyl-CoA used (C20:4, C18:2, C18:1, C16:1, C18:0 or C16:0), and were in the range of 9–17 μM, and 35–74 pmol/min/mg protein for E2, and in the range of 45–120 μM, and 30–182 pmol/min/mg protein for DHEA. Kinetic parameters were also assessed in gonadal tissue. The enzyme saturated at similar concentrations, although conjugation rates were lower than in digestive gland. Preliminary data shows that tributyltin (TBT) in the low μM range (1–50) strongly inhibits E2 and DHEA esterification, the esterification of E2 being more sensitive to inhibition than that of DHEA. Overall, results indicate that apolar conjugation occurs in oysters, in both digestive gland and gonads, at a very similar rate to mammals, suggesting that this is a well conserved conjugation pathway during evolution. Esterification, together with other mechanisms, can modulate endogenous steroid levels in C. virginica, and might be a target for endocrine disrupters, such as TBT.
Descripción4 pages, 1 table.-- PMID: 15178069 [PubMed].-- Printed version published in issue Aug-Dec 2004.-- Issue title: "Twelfth International Symposium on Pollutant Responses in Marine Organisms" (Tampa Bay, FL, United States, May 9-13, 2003).
Versión del editorhttp://dx.doi.org/10.1016/j.marenvres.2004.03.032
URIhttp://hdl.handle.net/10261/17648
DOI10.1016/j.marenvres.2004.03.032
ISSN0141-1136 (Print)
1879-0291 (Online)
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