English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/176347
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Whole genome sequencing-based analysis of tuberculosis (TB) in migrants: rapid tools for cross-border surveillance and to distinguish between recent transmission in the host country and new importations

AuthorsAbascal, Estefanía; Pérez-Lago, Laura; Martínez-Lirola, Miguel; Chiner-Oms, Álvaro ; Herranz, Marta; Chaoui, Imane; Comas, Iñaki ; El Messaoudi, My Driss; Garrido Cárdenas, José Antonio; Santantón, Sheila; Bouza, Emilio; García de Viedma, Darío
Cross-border surveillance
Molecular epidemiology
Whole genome sequencing
Issue DateJan-2019
PublisherEuropean Centre for Disease Prevention and Control
CitationEuro Surveillance 24(4): Art.1800005 (2019)
AbstractBackgroundThe analysis of transmission of tuberculosis (TB) is challenging in areas with a large migrant population. Standard genotyping may fail to differentiate transmission within the host country from new importations, which is key from an epidemiological perspective.AimTo propose a new strategy to simplify and optimise cross-border surveillance of tuberculosis and to distinguish between recent transmission in the host country and new importationsMethodsWe selected 10 clusters, defined by 24-locus mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR), from a population in Spain rich in migrants from eastern Europe, north Africa and west Africa and reanalysed 66 isolates by whole-genome sequencing (WGS). A multiplex-allele-specific PCR was designed to target strain-specific marker single nucleotide polymorphisms (SNPs), identified from WGS data, to optimise the surveillance of the most complex cluster.ResultsIn five of 10 clusters not all isolates showed the short genetic distances expected for recent transmission and revealed a higher number of SNPs, thus suggesting independent importations of prevalent strains in the country of origin. In the most complex cluster, rich in Moroccan cases, a multiplex allele-specific oligonucleotide-PCR (ASO-PCR) targeting the marker SNPs for the transmission subcluster enabled us to prospectively identify new secondary cases. The ASO-PCR-based strategy was transferred and applied in Morocco, demonstrating that the strain was prevalent in the country.ConclusionWe provide a new model for optimising the analysis of cross-border surveillance of TB transmission in the scenario of global migration.
Description14 páginas, 7 figuras
Publisher version (URL)http://dx.doi.org/ 10.2807/1560-7917.ES.2019.24.4.1800005
Appears in Collections:(IBV) Artículos
Files in This Item:
File Description SizeFormat 
2019 Euro Surveill 24-1800005.pdf897,27 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.