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Título

Nectar Flavonol Rhamnosides Are Floral Markers of Acacia (Robinia pseudacacia) Honey

AutorTruchado, Pilar ; Ferreres, Federico ; Bortolotti, Laura; Sabatini, Anna Gloria; Tomás Barberán, Francisco
Palabras claveFlavonoids
Floral markers
Botanical origin
Floral nectar
Robinia
Honey quality
HPLC-MS-MS
Fecha de publicación27-ago-2008
EditorAmerican Chemical Society
CitaciónJournal of Agricultural and Food Chemistry 56(19): 8815–8824 (2008)
ResumenWith the objective of finding floral markers for the determination of the botanical origin of acacia (robinia) honey, the phytochemicals present in nectar collected from Robinia pseudacacia flowers were analyzed by high-performance liquid chromatography−tandem mass spectrometry. Eight flavonoid glycosides were detected and characterized as kaempferol combinations with rhamnose and hexose. Acacia honey produced in the same location where the nectar was collected contained nectar-derived kaempferol rhamnosides. This is the first time that flavonoid glycosides have been found as honey constituents. Differences in the stability of nectar flavonoids during honey elaboration and ripening in the hive were shown to be due to hydrolytic enzymatic activity and to oxidation probably related to hydrogen peroxide (glucose-oxidase) activity. Acacia honeys contained propolis-derived flavonoid aglycones (468−4348 μg/100 g) and hydroxycinnamic acid derivatives (281−3249 μg/100 g). In addition, nectar-derived kaempferol glycosides were detected in all of the acacia honey samples analyzed (100−800 μg/100 g). These flavonoids were not detected in any of the different honey samples analyzed previously from different floral origins other than acacia. Finding flavonoid glycosides in honey related to floral origin is particularly relevant as it considerably enlarges the number of possible suitable markers to be used for the determination of the floral origin of honeys.
Descripción10 pages, 4 tables, 9 figures.
Versión del editorhttp://dx.doi.org/10.1021/jf801625t
URIhttp://hdl.handle.net/10261/17526
DOI10.1021/jf801625t
ISSN0021-8561 (Print)
1520-5118 (Online)
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