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Title

UCYN‐A3, a newly characterized open ocean sublineage of the symbiotic N2‐fixing cyanobacterium Candidatus Atelocyanobacterium thalassa

AuthorsCornejo-Castillo, Francisco M. ; Muñoz-Marín, Maria del Carmen; Turk-Kubo, Kendra; Royo-Llonch, Marta ; Farnelid, Hanna; Acinas, Silvia G. ; Zehr, Jonathan P.
Issue DateJan-2019
PublisherSociety for Applied Microbiology
CitationEnvironmental Microbiology 21(1): 111-124 (2018)
AbstractThe symbiotic unicellular cyanobacterium Candidatus Atelocyanobacterium thalassa (UCYN-A) is one of the most abundant and widespread nitrogen (N2 )-fixing cyanobacteria in the ocean. Although it remains uncultivated, multiple sublineages have been detected based on partial nitrogenase (nifH) gene sequences, including the four most commonly detected sublineages UCYN-A1, UCYN-A2, UCYN-A3 and UCYN-A4. However, very little is known about UCYN-A3 beyond the nifH sequences from nifH gene diversity surveys. In this study, single cell sorting, DNA sequencing, qPCR and CARD-FISH assays revealed discrepancies involving the identification of sublineages, which led to new information on the diversity of the UCYN-A symbiosis. 16S rRNA and nifH gene sequencing on single sorted cells allowed us to identify the 16S rRNA gene of the uncharacterized UCYN-A3 sublineage. We designed new CARD-FISH probes that allowed us to distinguish and observe UCYN-A2 in a coastal location (SIO Pier; San Diego) and UCYN-A3 in an open ocean location (Station ALOHA; Hawaii). Moreover, we reconstructed about 13% of the UCYN-A3 genome from Tara Oceans metagenomic data. Finally, our findings unveil the UCYN-A3 symbiosis in open ocean waters suggesting that the different UCYN-A sublineages are distributed along different size fractions of the plankton defined by the cell-size ranges of their prymnesiophyte hosts
Description14 pages, 4 figures, 1 table, supporting information https://doi.org/10.1111/1462-2920.14429
Publisher version (URL)https://doi.org/10.1111/1462-2920.14429
URIhttp://hdl.handle.net/10261/175073
Identifiersdoi: 10.1111/1462-2920.14429
issn: 1462-2912
e-issn: 1462-2920
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