English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/174850
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorEncinar del Dedo, Javieres_ES
dc.contributor.authorIdrissi, Fátima-Zahraes_ES
dc.contributor.authorGarcia, Patriciaes_ES
dc.contributor.authorFernández-Golbano, Isabel Maríaes_ES
dc.contributor.authorRebollo, Elenaes_ES
dc.contributor.authorKrzyzanowski, Marek K.es_ES
dc.contributor.authorGrötsch, Helgaes_ES
dc.contributor.authorGeli, María Isabeles_ES
dc.identifier.citationCNRS - Conférences Jacques Monod (2017)es_ES
dc.descriptionTrabajo presentado en el CNRS - Conférences Jacques Monod: “Molecular basis for membrane remodelling and organization”, celebrado en Roscoff (Francia), del 3 al 7 de abril de 2017es_ES
dc.description.abstractOxysterol Binding Proteins (OSBP) are a family of conserved lipid binding proteins, enriched at endoplasmic reticulum (ER) contacts sites. OSBPs promote non-vesicular lipid transport to other organelles and work as lipid sensors in the context of multiple cellular tasks, but the determinants of their distinct localization and function are still not understood. Using a combination of Time Resolved Electron Microscopy (TREM) and life-cell imaging in yeast, we demonstrate that the endocytic invaginations associate with the cortical endoplasmic reticulum as they mature, and that this association requires the OSBPs Osh2 and Osh3, which bridge the endocytic myosin-I Myo5 to the ER integral-membrane VAMP-associated protein (VAP) Scs2. Using mutations that specifically disrupt the myosin-I/OSBP/VAP link, as well as a reticulon mutant with extended cER-free plasma membrane subdomains, we show that ER contact to the endocytic sites has a dual function initiating actin-dependent membrane invagination and promoting vesicle scission. Further, we show that ER-induced actin polymerization requires the localized transfer of sterols by the endocytic OSBPs.es_ES
dc.titleOSBP-mediated ER contact with endocytic sites initiates actin polymerizationes_ES
dc.typecomunicación de congresoes_ES
oprm.item.hasRevisionno ko 0 false*
Appears in Collections:(IBMB) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show simple item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.