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Distinct Dual C-Cl Isotope Fractionation Patterns during Anaerobic Biodegradation of 1,2-Dichloroethane: Potential to Characterize Microbial Degradation in the Field

AuthorsPalau, Jordi; Yu, Rong; Hatijah Mortan, Siti; Shouakar- Stash, Orfan; Rosell, Mònica; Freedman, David L.; Sbarbati, Chiara; Fiorenza, Stephanie; Aravena, Ramón; Marco-Urrea, Ernest; Elsner, Martin; Soler, Albert; Hunkeler, Daniel
KeywordsAerobic bacteria
Microbial degradation
Issue Date7-Mar-2017
PublisherAmerican Chemical Society
CitationEnvironmental Science and Technology 51 (5): 2685-2694 (2017)
AbstractThis study investigates, for the first time, dual C-Cl isotope fractionation during anaerobic biodegradation of 1,2-dichloroethane (1,2-DCA) via dihaloelimination by Dehalococcoides and Dehalogenimonas-containing enrichment cultures. Isotopic fractionation of 1,2-DCA (εbulkC and εbulkCl) for Dehalococcoides (-33.0 ± 0.4‰ and -5.1 ± 0.1‰) and Dehalogenimonas-containing microcosms (-23 ± 2‰ and -12.0 ± 0.8‰) resulted in distinctly different dual element C-Cl isotope correlations (∇ = Δδ13C/Δδ37Cl ≈ εbulkC/εbulkCl), 6.8 ± 0.2 and 1.89 ± 0.02, respectively. Determined isotope effects and detected products suggest that the difference on the obtained ∇ values for biodihaloelimination could be associated with a different mode of concerted bond cleavage rather than two different reaction pathways (i.e., stepwise vs concerted). ∇ values of 1,2-DCA were, for the first time, determined in two field sites under reducing conditions (2.1 ± 0.1 and 2.2 ± 2.9). They were similar to the one obtained for the Dehalogenimonas-containing microcosms (1.89 ± 0.02) and very different from those reported for aerobic degradation pathways in a previous laboratory study (7.6 ± 0.1 and 0.78 ± 0.03). Thus, this study illustrates the potential of a dual isotope analysis to differentiate between aerobic and anaerobic biodegradation pathways of 1,2-DCA in the field and suggests that this approach might also be used to characterize dihaloelimination of 1,2-DCA by different bacteria, which needs to be confirmed in future studies. © 2017 American Chemical Society.
Publisher version (URL)10.1021/acs.est.6b04998
Appears in Collections:(IDAEA) Artículos
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