English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/174423
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D

AuthorsArroyo, R.; Martín-González, A.; Echaide, M.; Jain, A.; Brondyk, W.H.; Rosenbaum, J.; Moreno-Herrero, Fernando; Pérez-Gil, J.
Lung surfactant
Non-covalent interactions
Issue DateMay-2018
PublisherAcademic Press
CitationJournal of Molecular Biology 430 (10): 1495-1509 (2018)
AbstractPulmonary surfactant protein D (SP-D) is a glycoprotein from the collectin family that is a component of the lung surfactant system. It exhibits host defense and immune regulatory functions in addition to contributing to the homeostasis of the surfactant pool within the alveolar airspaces. It is known that the SP-D monomer forms trimers, which further associate into higher-order oligomers. However, the pathway and the interactions involved in the assembly of SP-D oligomers are not clearly understood. In the current study, a recombinant form of full-length human SP-D (rhSP-D) has been qualitatively and quantitatively studied by atomic force microscopy (AFM) and electrophoresis, with the aim to understand the conformational diversity and the determinants defining the oligomerization of the protein. The rhSP-D preparation studied is a mixture of trimers, hexamers, dodecamers and higher-order oligomeric species, with dodecamers accounting for more than 50% of the protein by mass. Similar structures were also found in hSP-D obtained from proteinosis patients, with the largest fuzzy-ball-like oligomers being more abundant in these samples. The proportion of dodecamer is increased under acidic conditions, accompanied by a conformational change into more compact configurations. Two hexamers appear to be the minimal necessary unit for dodecamer formation, with stabilization of the dodecamer occurring via non-covalent, ionic, and hydrophobic interactions between the individual N-terminal domains and the proximal area of the SP-D collagen stems.
Publisher version (URL)http://dx.doi.org/10.1016/j.jmb.2018.03.027
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
Arroyo et al JMolBiol 2018 v3 final text marked.docx164,68 kBMicrosoft Word XMLView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.